An Alternative Approach to Time-consuming Plasma Stripping for Endogenous Compounds: Application to Progesterone Determination by LC/MS/MS
PRESENTED TO: BioPharma Services Inc.
PRESENTED BY: A. Tan, K. P. Hang, J. K. Patel, K. Awaiye, F. Trabelsi
PURPOSE
An alternative approach to time-consuming plasma stripping for endogenous compounds:application to progesterone determination by LC/MS/MS – It is always challenging to obtain an appropriate analyte-free matrix for the preparation of calibration curve for endogenous compounds. One of the most common approaches is charcoal-stripping. Though it is effective in many cases, the process is not only time-consuming, but also difficult to control (the extent of stripping). Moreover, some matrix components could be removed, reducing the similarity of the stripped matrix to a non stripped one. The purpose of this research was to find a more efficient and easier-to-control-approach and to apply it to the quantitation of progesterone in human plasma by LC/MS/MS.
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